GREEN SYNTHESIS CHARACTERIZATION AND ANTIMICROBIAL ACTIVITIES OF SILVER NANOPARTICLES USING LEAVES EXTRACT OF PROSOPIS CINERARIA

Abstract

Present work represents ecofriendly green approach for the synthesis of silver nanoparticles using leaf extract of Prosopis cineraria and salt solution of silver nitrate. The synthesized nanoparticles were characterized using ultraviolet visible (UV-Vis), Fourier transform infrared (FT-IR), X-Rays diffraction (XRD) crystalline nature of silver nanoparticles and calculated size was 25 nm, high-resolution transmission electron microscopy (HRTEM), and scanning electron microscopy (SEM). techniques. High-resolution transmission electron microscopic analysis substantiated that the AgNPs were rod-shaped with an average size of 38 nm. SEM analysis revealed that the AgNPs were crystalline rod-shaped with porous morphology. Our SEM findings indicated that the size range of the produced silver nanoparticles exceeded the typical nanoparticle size range due to agglomeration, which should normally be between 25 to 28 nanometers. The proteins that surrounded and were bound to the surface of the produced silver nanoparticles caused their size range to be greater than the preferred size. X-ray diffraction study revealed that the AgNPs were in the face-centered cubic crystal system. The hydroxyl groups of Prosopis cineraria leaf extract acted as reducing agents, which was corroborated by the broad peak infrared spectrum 3400 cm-1. Peak at 3400-1 and a ester carbonyl peak near 1750 cm-1. The silver nanoparticles were confirmed by the presence of a very small peak 2300-1 of peaks along with two other peaks at 1615 cm-1 and 1320 cm-1. The antibacterial activities of silver nanoparticles inhibited the growth of bacterium that showed inhibition zone of 15 nm against Bacillus subtilis, and standard ciprofloxacin showed inhibition zone of 18 nm against the same bacterium.  Salmonella typhi showed inhibition zone of 19 nm and standard drug ciprofloxacin showed inhibition zone of 21 nm against the same bacterium.

Keywords : Prosopis cineraria, nickel oxide, nanoparticles, green synthesis, Salmonella typhi,  Bacillus subtilis